The Effect and Mechanism of KLF7 in the TLR4/NF-κB/IL-6 Inflammatory Signal Pathway of Adipocytes

Figure 1

The mRNA expressions of TLR4, KLF7, and inflammatory cytokines (a–f) in adipocytes and the IL-6 protein release level (g), which were treated with 0, 0.2, 2, 20, 100, and 200 μmol/L PA. The protein expression of TLR4, KLF7, and inflammatory cytokines in adipocytes was stimulated by 100 μmol/L PA (j). Differentiation of 3T3-L1 preadipocytes into mature adipocytes, and Oil Red O staining of lipid droplets as viewed under a microscope at (h) 200x and (i) 400x magnification. Grayscale scanning of proteins (k). The data for each group is derived from three replicates of experimental data. One-way ANOVA (a, c–g), Kruskal-Wallis H test (b), T test (k), ; the difference was statistically significant.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)