The Effect and Mechanism of KLF7 in the TLR4/NF-κB/IL-6 Inflammatory Signal Pathway of Adipocytes

Figure 3

Detection of KLF7 mRNA expression at 24 h and 48 h after transfection of mature adipocytes with 3 μg/mL KLF7 overexpression plasmid (a). The expression of KLF7 protein was further detected after 48 h of transfection (b, c). The mRNA and protein expression of inflammatory factors in adipocytes after 48 h transfection with KLF7 overexpression plasmids (d–i). Grayscale scanning of (b) and (i) proteins (c, j) (Ad-KLF7: transfected KLF7 overexpression plasmid; Ad-NC: transfected KLF7 negative control overexpression plasmid). The data for each group is derived from three replicates of experimental data. One-way ANOVA (a), T test (c, d, f–h, j), Wilcoxon rank sum test (e), Ad-NC: Ad-KLF7, , ; the difference was statistically significant.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)