Anti-TNF-α Therapy Suppresses Proinflammatory Activities of Mucosal Neutrophils in Inflammatory Bowel Disease

Figure 2

The activity of neutrophils is inhibited in the peripheral blood, and the infiltration of neutrophils is decreased in the intestinal mucosa of CD patients after anti-TNF-α treatment. Response group: (a) percentages of CD66b⁺ neutrophils in the peripheral blood of patients with active CD () before and after IFX treatment; (b) expression of CD66b mRNA in the peripheral blood neutrophils from patients with active CD () before and after IFX treatment by qRT-PCR; (c) expression of CD66b mRNA in intestinal mucosa from patients with active CD () before and after IFX treatment by qRT-PCR; (d) immunohistochemical staining of CD66b in the intestinal mucosa of active CD () before and after IFX treatment; and (e) expression of S100A8, (f) S100A9, and (g) MPO in the intestinal mucosa of patients with active CD () before and after IFX treatment. Failure group: percentage (h) and expression of CD66b (i) of peripheral blood neutrophils from active CD patients () before and after IFX. Expression of CD66b (j), S100A8 (k), S100A9 (l), and MPO (m) in intestinal mucosa was detected by qRT-PCR from CD patients in the failure group. , , and .

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