Involvement of M1 Macrophage Polarization in Endosomal Toll-Like Receptors Activated Psoriatic Inflammation

Figure 2

Depletion of macrophage attenuates imiquimod- (IMQ-) induced psoriasis-like inflammation. (a) Balb/c mice were intraperitoneally injected with clodronate-containing liposomes and topically treated with IMQ cream following the schedule illustrated. (b) The severity of inflammatory responses on the skin was assessed on the basis of the Psoriasis Area Severity Index. (c) Five days after IMQ treatment, the mice were sacrificed and their skin thickness was measured with vernier caliper to assess the severity of the psoriatic responses. (d, e) Tissue samples from the control and IMQ-treated mice were analyzed using quantitative real-time polymerase chain reaction for the expression of (d) M1 and M2 macrophage markers and (e) inflammatory cytokines and chemokines. The data represent mean ± standard deviation (); and compared with the controls, or as indicated.

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