A Profibrotic Phenotype in Naïve and in Fibrotic Lung Myofibroblasts Is Governed by Modulations in Thy-1 Expression and Activation

Figure 1

Thy1 gene expression following genetic manipulation, using Thy1 knockout mice, Thy1 siRNA, and Thy1 cDNA expression vector. (a) Flow cytometry Thy-FITC mAb staining in spleen cells from wild-type (WT) C57BL/6 mice and Thy1-deficient mice, respectively. (b) Downregulation of Thy1 by siRNA was performed. Thy1 downregulation was detected 48 h after transfection by FACS analysis (c) Mlg cells were transfected with Thy1 expression vector. Plasmid-containing Thy1 cDNA in antisense orientation serves as a control. Thy1 expression was detected 24 h after the transfection by FACS analysis.

(a)

(b)

(c)