A Profibrotic Phenotype in Naïve and in Fibrotic Lung Myofibroblasts Is Governed by Modulations in Thy-1 Expression and Activation

Figure 8

Thy1 expression is involved in the regulation of collagen in fibroblasts. QPCR (a) and Western blots (b-c) determining the extent of collagen expression in Thy1⁺ (a) or Thy1⁻ (b) sorted fibroblasts from the lungs of bleomycin-treated mice and in (c) naïve lung fibroblast primary cultures following stimulation with G7 anti-Thy1 mAb (5 μg/ml) or IgG isotype match control for 24–72 h.

(a)

(b)

(c)