Neuropeptides SP and CGRP Diminish the Moraxella catarrhalis Outer Membrane Vesicle- (OMV-) Triggered Inflammatory Response of Human A549 Epithelial Cells and Neutrophils

Figure 3

Neuropeptide-mediated abrogation of cytotoxic effect of M. catarrhalis OMVs on A549 human epithelial cells. The confluent cells were exposed to the indicated concentrations of OMVs (protein concentration) over 24 h of incubation, and the cell viability (% of untreated control) was determined using the MTT assay. (a) Cytotoxic effect of indicated OMVs (protein concentration) and 400 μM 3-BP (3-bromopyruvate) as positive control. (b) Anticytotoxic effect of physiological concentrations (10⁻⁸ M) of neuropeptides towards cell death induced by OMVs at 100 μg/ml. Data are expressed as the of three independent experiments performed at least in four replicates. Statistics was performed by one-way ANOVA followed by Tukey’s post hoc test. (a) compared to untreated control and (b) compared to OMV treatment.

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