Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model

Figure 2

Survival of animals during the CLP procedure. The cumulative survival of animals that underwent the CLP procedure or sham-operated control animals up until 14 days following the procedure. In (a), the cecum was ligated at 75% of its length, whereas in (b), it was ligated at 50% of its length. Note the influence of the needle thickness on survival in (a), with the large bore needles resulting in a higher mortality rate. When ligating 50% of the cecum’s length, no mortality was observed when only one puncture was performed independent of the needle size (b). The needle size was then again of influence once two punctures were performed. animals/group. CLP: cecal ligation and puncture; interpretation of the numeric code used following “CLP”: the first two numbers represent the percentage of the cecum that was ligated, the third number represents how many times the cecum was punctured, and the final two numbers represent the size of the needle, for example, CLP50125 means CLP procedure in which the cecum is ligated at 50% of its length and punctured once (1) with a 25G needle.

(a)

(b)