Inflammation and LPS-Binding Protein Enable the Stimulatory Effect of Endotoxin on Prolactin Secretion in the Ovine Anterior Pituitary: Ex Vivo Study

Table 1

Specific primers used in real-time PCR analysis to determine the expression of housekeeping genes and genes of interest.

Gene	GeneBank acc. number	Amplicon size (bp)	Sequence 5→ 3
			Forward	Reverse
GAPDH^a	NM_001034034	134	AGAAGGCTGGGGCTCACT	GGCATTGCTGACAATCTTGA
ACTB^a	U39357	168	CTTCCTTCCTGGGCATGG	GGGCAGTGATCTCTTTCTGC
HDAC1^a	BC108088.1	115	CTGGGGACCTACGGGATATT	GACATGACCGGCTTGAAAAT
PRb^b	NM_001009306	131	CCTCTCCTCGGAAATGTTCA	AGGACTTCATGGTGGGTCTG
TLR4^c	AY957615	117	GGTTCCCAGAACTGCAAGTG	GGATAGGGTTTCCCGTCAGT
IL1B^a	X54796.1	137	CAGCCGTGCAGTCAGTAAAA	GAAGCTCATGCAGAACACCA
IL6^a	NM_001009392.1	165	GTTCAATCAGGCGATTTGCT	CCTGCGATCTTTTCCTTCAG
TNF^a	NM_001024860	153	CAAATAACAAGCCGGTAGCC	AGATGAGGTAAAGCCCGTCA

References: ^a[39]; ^b[21]; ^c[40].