RIG-I Signaling via MAVS Is Dispensable for Survival in Lethal Influenza Infection In Vivo

Figure 3

Virus PRR mRNA induction in RIG-I KO mice during IAV infection. (a) mRNA induction in mouse lung. Mice were infected with 300 pfu of the IAV PR8 strain. Mock-treated mice were inoculated with PBS. At the indicated time points, the mice were sacrificed and lung tissues were collected for RNA preparation. (b) mRNA induction in primary ear fibroblasts infected with IAV. Primary ear fibroblasts isolated from RIG-I WT and KO mice were infected with IAV PR8 at an MOI of 1. After 24 h, cells were collected for RNA extraction. The mRNA levels were assessed by qRT-PCR and normalized by β-actin. Data are expressed as of fold increase over the WT mock group ( per group). For clarity, we only show significant differences () between the PR8-infected RIG-I KO group and the PR8-infected WT group. value was calculated from the ΔΔCt values from different experimental groups.

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