RIG-I Signaling via MAVS Is Dispensable for Survival in Lethal Influenza Infection In Vivo

Figure 6

MAVS is dispensable for survival and cytokine induction during influenza infection. MAVS KO and littermate WT mice were intranasally inoculated with IAV at 1000 pfu/mouse. Mortality (a) and body weights (b) were monitored daily. Body weight data were normalized to each mouse’s starting body weight. Data are expressed as deviation ( for MAVS KO mice; for WT mice). (c) PRR and cytokine mRNA induction in mouse lung. Mice were infected with 300 pfu of the IAV PR8 strain. Mock-treated mice were inoculated with PBS. At day 6 postinfection, the mice were sacrificed and lung tissues were collected for RNA preparation. The mRNA levels were assessed by qRT-PCR and normalized β-actin. Data are expressed as of fold increase ( per group). For clarity, we only show significant differences () between the PR8-infected MAVS KO group and the PR8-infected WT group. value was calculated from the ΔΔCt values from different experimental groups.

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