Royal Jelly Attenuates LPS-Induced Inflammation in BV-2 Microglial Cells through Modulating NF-κB and p38/JNK Signaling Pathways

Figure 2

Effects of RJ on LPS-induced production of NO and ROS, as well as the protein expression of iNOS and COX-2 in BV-2 cells. BV-2 cells were pretreated with RJ (0, 0.3, 1, and 3 mg/mL) for 1 h, followed by 24 hours of incubation with LPS (1 μg/mL). The NO production in cell supernatants was detected by Griess reaction (a), and intracellular ROS levels were measured using DCF fluorescence as described in the text (b). BV-2 cells were pretreated in the same way as described above and were then stimulated with 1 μg/mL LPS for 24 h. Proteins were extracted, and Western blot analysis was conducted using specific antibodies against iNOS and COX-2 (c–e). β-Tubulin protein was used here as an internal control. Data are presented as means ± SEM, and group differences were analyzed by one-way ANOVA with post hoc Tukey’s test. compared with untreated control group; , compared with the group treated with LPS alone.

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