Royal Jelly Attenuates LPS-Induced Inflammation in BV-2 Microglial Cells through Modulating NF-κB and p38/JNK Signaling Pathways

Figure 3

Effects of RJ on LPS-induced mRNA expression of inflammatory mediators in BV-2 microglial cells. BV-2 cells were preincubated in the culture medium with RJ (0, 0.3, 1, and 3 mg/mL) for 1 h and were then stimulated with 1 μg/mL LPS for another 6 h. BV-2 cells were harvested for RNA extraction with Trizol, and the mRNA expressions of iNOS (a), COX-2 (b), MCP-1 (c), IL-6 (d), TNF-α (e), IL-1β (f), and HO-1 (g) were measured by qRT-PCR. Data are presented as means ± SEM, and group differences were analyzed by one-way ANOVA with post hoc Tukey’s test. compared with untreated control group; , compared with the group treated with LPS alone.

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