Royal Jelly Attenuates LPS-Induced Inflammation in BV-2 Microglial Cells through Modulating NF-κB and p38/JNK Signaling Pathways

Figure 5

Effects of RJ on LPS-induced activation of NF-κB and MAPK pathways. BV-2 cells were pretreated with RJ (0, 0.3, 1, and 3 mg/mL) for 1 h, followed by a 45 min incubation with 1 μg/mL LPS. NF-κB p65 nucleus translocation was observed by confocal laser microscope, scale bar = 10 μm (a). BV-2 cells were pretreated in the same way as described above. Proteins were extracted, and Western blot analysis was conducted using specific antibodies (b–f). β-Tubulin protein was used as an internal control. Data are presented as means ± SEM, and group differences were analyzed by one-way ANOVA with post hoc Tukey’s test. compared with untreated control group; , compared with the group treated with LPS alone.

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