Research Article
Royal Jelly Attenuates LPS-Induced Inflammation in BV-2 Microglial Cells through Modulating NF-κB and p38/JNK Signaling Pathways
Table 1
Primer sequence used in qRT-PCR.
| | Gene | Forward primer | Reverse primer | Product size (bp) | GenBank accession number |
| | IL-6 | 5-CTCTGCAAGAGACTTCCATCC-3 | 5-GAATTGCCATTGCACAACTC-3 | 210 | NM_031168.1 | | iNOS | 5-TTTCCAGAAGCAGAATGTGACC-3 | 5-AACACCACTTTCACCAAGACTC-3 | 294 | NM_010927.3 | | COX-2 | 5-GAAATATCAGGTCATTGGTGGAG-3 | 5-GTTTGGAATAGTTGCTCATCAC-3 | 237 | NM_011198.3 | | TNF-α | 5-CCACGCTCTTCTGTCTACTG-3 | 5-ACTTGGTGGTTTGCTACGAC-3 | 169 | NM_013693.2 | | HO-1 | 5-ACATTGAGCTGTTTGAGGAG-3 | 5-TACATGGCATAAATTCCCACTG-3 | 241 | NM_010442.2 | | MCP-1 | 5-AAGAAGCTGTAGTTTTTGTCACCA-3 | 5-TGAAGACCTTAGGGCAGATGC-3 | 155 | NM_011333.3 | | IL-1β | 5-ATCTCGCAGCAGCACATCAAC-3 | 5-TGTTCATCTCGGAGCCTGTAGT-3 | 239 | NM_008361.3 | | GAPDH | 5-GAGAAACCTGCCAAGTATGATGAC-3 | 5-TAGCCGTATTCATTGTCATACCAG-3 | 212 | NM_008084.2 |
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