Serotonin Exhibits Accelerated Bleomycin-Induced Pulmonary Fibrosis through TPH1 Knockout Mouse Experiments

Figure 6

Effect of 5-HT on neutrophil infiltration and oxidative stress induced by bleomycin in mice. Intratracheal instillation of bleomycin (BLM) hydrochloride was performed to induce pulmonary fibrosis in wild-type C57BL/6 (WT) and TPH1 knockout (KO) mice. The mice in the WT and KO control, WT BLM, and KO BLM groups were sacrificed on day 21. Lung tissues were collected to perform immunofluorescent staining to detect the location of (a) myeloperoxidase (MPO) and the calculation of (b) MPO positively stained cells. Meanwhile, the lung (c) malondialdehyde (MDA), (d) superoxide dismutase (SOD), and (e) glutathione (GSH) levels were examined to determine the severity of oxidative stress. All data were expressed as , .

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