CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages

Figure 3

CD38 deficiency suppresses NF-κB expression in macrophages. The protein levels of Sirt1 and NF-κB p65 were determined by Western blot in primary macrophages (a) and RAW264.7 cells (b). The different clones of RAW264.7 cells, including the negative control dCas9 and the CD38 knockdown cell line E1a and E3, were selected for the experiment. The GAPDH was used as an internal control. The data represent the mean ± S.D. from three independent experiments, , compared with the WT group.

(a)

(b)