How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation

Figure 1

Migration of polymorphonuclear neutrophils (PMNs) into the different compartments of the lung (a). LPS inhalation caused an increase of PMNs adherent to the pulmonary endothelium (ADHERENT), in the lung interstitium (IS), and the alveolar space (BAL) after the inflammation (). Impact of inflammation on the expression of adhesion molecules in the lungs of mice (b). The expression of the adhesion molecules were investigated after the onset of inflammation by RT-PCR (). Data are presented as mean ± SD; ; ; as indicated. Determination of adhesion molecule expression by immunofluorescence (c). Mice were treated with LPS and three representative adhesion molecules investigated (). Images are representatives of four experiments with similar results (original magnification, ×63). The particular adhesion molecule was stained with a specific antibody and appears green, nuclei were stained with DAPI and emerge blue, and polymorphonuclear leukocytes (PMN) appear red.

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