Effect of Propofol on the Production of Inflammatory Cytokines by Human Polarized Macrophages

Figure 2

Propofol reduced IL-6 and IL-1β gene expression and protein production in M1 macrophages. M0 macrophages were polarized to M1 macrophages in the presence of 0.05% DMSO (solvent control) or propofol (1–5 μM). (a–c) qRT-PCR assays of IL-6, IL-1β, and TNF-α mRNA levels in M1 macrophages. Data were normalized relative to β-actin mRNA (internal control) and presented as ( per group). (d–f) ELISA measurements of IL-6 (d), IL-1β (e), and TNF-α (f) secreted by M1 macrophages. Data are presented as ( per group). , , and compared with control cells by one-way ANOVA with Bonferroni’s post hoc test.

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