Effect of Propofol on the Production of Inflammatory Cytokines by Human Polarized Macrophages

Figure 3

Muscimol reduced IL-6 and IL-1β gene expression and protein production in M1 macrophages. M0 macrophages were polarized to M1 macrophages in the absence or presence of muscimol (100 μM). (a–d) qRT-PCR assays of IL-6, IL-1β, TNF-α, and CD86 mRNA. Data were normalized relative to β-actin mRNA (internal control) and presented as ( per group). (e–g) ELISA measurements of IL-6 (e), IL-1β (f), and TNF-α (g) secreted by M1 macrophages. Data are presented as ( per group). compared with control cells by Wilcoxon-Mann-Whitney test.

(a)

(b)

(c)

(d)

(e)

(f)

(g)