Remifentanil Protects against Lipopolysaccharide-Induced Inflammation through PARP-1/NF-κB Signaling Pathway

Figure 1

Remifentanil reduced production and DNA damage in HAECs. After pretreatment with PBS or remifentanil for 30 min, HAECs were stimulated by LPS for 24 h, superoxide anion production was measured by dihydroethidium (DHE) and DNA damage was determined by comet assay. (a, b) production (red) was assessed by DHE. Nuclei were labelled with 4,6-diamidino-2-phenylindole (DAPI) (blue). . (c, d) The content of DNA in the tail was assessed by comet assay. . Values are . versus control. ^# versus LPS+PBS. HAECs: human aortic endothelial cells; LPS: lipopolysaccharide.

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