TGF-β3 Induces Autophagic Activity by Increasing ROS Generation in a NOX4-Dependent Pathway

Figure 6

NAC inhibits autophagy activation and MUC5AC expression in asthma mice models. (a) Schematic diagram of the experimental protocol for sensitization and challenge with HDM and the experimental protocol for the mice pretreated with NAC ( mice for each group). (b) Representative confocal laser immunofluorescence photomicrography of the lung tissues in the mice showed the ROS generation in the lung tissues. (c) The fold change of ROS signal intensity is shown. (d) TGF-β3 in BALF was detected by ELISA. (e) Representative immunofluorescence images of MUC5AC expression in the airway epithelial cells of the mice. (f) Quantitation of the fluorescence intensity of MUC5AC. (g) The expression of LC3B was detected using western blot assays. (h) Relative changes in the density of LC3B II. Each point is an individual mouse. Data are presented as , , and , determined by one-way ANOVA with Tukey-Kramer posttest.

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