Concomitant Treatment with Etanercept and Tacrolimus Synergistically Attenuates Arthritis Progression via Inhibition of Matrix Metalloproteinase-3 Production and Osteoclastogenesis in Human TNF-α Transgenic Mice

Table 1

Etanercept with concomitantly administered tacrolimus attenuates the inflammatory cell infiltration into the synovial lining in hTNF-Tg mice.

Parameter	Vehicle	ETN	TAC	ETN+TAC
Bone destruction
Cartilage damage
Synovial hyperplasia
Infiltration of inflammatory cells

H&E-stained sections of the limb joints from hTNF-Tg mice were scored as follows: 0: no change; 1: very slight; 2: slight; 3: moderate; 4: severe, with a maximum score of 16 per mouse for each item. Data are representative of two independent experiments and presented as of five animals per group. compared with the vehicle-treated group (joint-ranking Dunnett’s multiple comparison test). ETN: etanercept; TAC: tacrolimus.