Concomitant Treatment with Etanercept and Tacrolimus Synergistically Attenuates Arthritis Progression via Inhibition of Matrix Metalloproteinase-3 Production and Osteoclastogenesis in Human TNF-α Transgenic Mice

Table 2

Etanercept induces ADA production in hTNF-Tg mice.

	Vehicle	ETN	TAC	ETN+TAC
Relative serum ADA level (O. D. at 450 nm)

Etanercept (ETN; 4 mg/kg) was administered subcutaneously twice a week for 4 weeks, and tacrolimus (TAC; 3 mg/kg) was administered orally once daily for 4 weeks. Relative amounts of serum ADAs against etanercept were measured at the end of the dosing period by ELISA. Data are representative of two independent experiments and are presented as of five animals per group. compared with the vehicle group (Steel’s test).