Suppressive Effects of GSS on Lipopolysaccharide-Induced Endothelial Cell Injury and ALI via TNF-α and IL-6

Figure 5

Effects of GSS on the LPS-induced activation of NF-κB in ECs. (a, b) GSS had a significant inhibitory effect on LPS-induced increase of P-P65 expression compared to the LPS-stimulated group. (c, d) The expression of P65 in the nucleus of ECs was measured by Western blot analysis. (e, f) P65 expression in the cytoplasm of ECs was measured by WB assay. (g) The nuclear translocation of NF-κB was analyzed using the confocal microscope (×200 magnification), which revealed that GSS effectively blocked LPS-induced P65 translocation into the nucleus of ECs. versus negative control. ^# versus the corresponding LPS treatment group.

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