miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus

Figure 4

miR-657 can targetly regulate FAM46C. (a) miR-657 specially recognizes the 3UTR of FAM46C. (b) Luciferase reporter assay shows FAM46C is the direct target of miR-657 (; representative data of three repeated tests). (c) Decreased mRNA expression of FAM46C in miR-657 mimics treated THP-1 macrophages (; representative data of three repeated determinations). (d) Decreased protein expression of FAM46C in miR-657 mimics treated THP-1 macrophages determined by western blot (representative picture of three repeated tests). (e) Elevated mRNA expression of FAM46C in miR-657 inhibitors treated THP-1 cells (; ). (f) Increased protein expression of FAM46C in miR-657 inhibitors treated macrophages (representative picture of three independent experiments).

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