miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus

Figure 5

miR-657 promotes macrophage polarization toward M1 via FAM46C. (a) mRNA expression of IL-12, TNF-α, Arginase 1, and CD206 in miR-657/FAM46C overexpressed THP-1 macrophages (compared with the control group, , ; compared with the miR-657 overexpressed (OE) group, ; ). (b) Expression of IL-12 and TNF-α in the cultural supernatant of THP-1 cells (compared with the control group, ; compared with the miR-657 OE group, ; ). (c) Western blot estimating the protein expression of Arginase 1 and CD206 in macrophages (representative picture of three repeated tests). (d) The proliferation status of miR-657/FAM46C overexpressed macrophages (compared with the control group, , ; compared with the miR-657 OE group, ; ). (e) Transwell assay estimating the migration of miR-657/FAM46C overexpressed THP-1 cells (compared with the control group, ; compared with the miR-657 OE group, ; ).

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