Thrombin Induces Secretion of Multiple Cytokines and Expression of Protease-Activated Receptors in Mouse Mast Cell Line

Figure 5

Expression of MERKs and phospho-MAPKs (including JNK, P38, and ERK1/2) in P815 cells at 0.5, 1, 2, and 4 h after stimulation of 0.2 U/ml thrombin. The expression of p-P38, p-JNK, and p-ERK1/2 MERK in P815 cells increased obviously at 2 and 4 h after incubation with 0.2 U/ml thrombin, but not in the level of total P38, JNK, and ERK1/2. Blots are standardized to α-tubulin or β-actin expression. ANOVA was performed. Multiple comparisons were applied to compare the difference between each group. indicates that the difference between the control group and the diverse concentration of thrombin was statistically significant (; ; ). (a) Expression of JNK and phospho-JNK, standardized by β-actin. (b) Expression of P38 and phospho-P38, standardized by α-tubulin. (c) Expression of ERK1/2 and phospho-ERK1/2, standardized by α-tubulin.

(a) Expression of JNK and p-JNK in P815 cell at 0.5, 1, 2, and 4 h after thrombin 0.2 U/ml treatment

(b) Expression of P38 and p-P38 in P815 cell at 0.5, 1, 2, and 4 h after thrombin 0.2 U/ml treatment

(c) Expression ERK1/2 and p-ERK1/2 in P815 cell at 0.5, 1, 2, and 4 h after thrombin 0.2 U/ml treatment