Inhibition of Triggering Receptor Expressed on Myeloid Cell-1 Alleviates Acute Gouty Inflammation

Figure 3

Specific TREM-1 inhibitor (LP17) inhibited MSU-induced proinflammatory cytokine release in THP-1 cells. (a) The level of TREM-1 mRNA was detected by RT-qPCR. (b) The level of TREM-1 protein was detected using Western blot. (c–f) Protein levels of IL-1β, TNF-α, MCP-1, IL-6, and IL-8 were measured by ELISA. Data were presented as the of three wells per group. Individual experiments were conducted three times. , compared to the blank group; , compared to the MSU group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)