Flemingia philippinensis Flavonoids Relieve Bone Erosion and Inflammatory Mediators in CIA Mice by Downregulating NF-κB and MAPK Pathways

Figure 5

FPF treatment inhibited bone degradation proteinase expression, and nuclear factor kappa-B (NF-κB) activation in the CIA mouse model. C57BL/6 mice were injected intradermally at the base of the tail with CII on day 0 and then challenged with 50 μg CII subplantar at both hind limbs on day 18. On day 46, paws were dissected and total proteins were extracted (-4 each group). Expressions of the indicated cathepsin K, MMP-9, phosphorylated (p-) I-κBα, and p-p65 in the paw were examined using western blot and quantified by densitometric analyses. All data are error of the mean (SEM) and and vs. the vehicle group as determined using Student’s -test.