Flemingia philippinensis Flavonoids Relieve Bone Erosion and Inflammatory Mediators in CIA Mice by Downregulating NF-κB and MAPK Pathways

Figure 7

FPF treatment inhibited proinflammatory protein expression and AP-1 and STAT3 activation in the CIA mouse model. C57BL/6 mice were injected intradermally with CII at the base of the tail on day 0 and then challenged with 50 μ g CII subplantar at both hind limbs on day 18. On day 46, the paws and the spleen were dissected and total proteins were extracted (-4 each group). Expressions of phosphorylated (p-) Jun D+c-Jun (a) and p-STAT3 (b) in paws and MCP-1 in the spleen (c) were determined using western blot and quantified by densitometric analyses. All data are error of the mean (SEM). and vs. the vehicle-treated control as determined using Student’s -test.

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