Research Article
Escherichia coli Nissle 1917 Protects Intestinal Barrier Function by Inhibiting NF-κB-Mediated Activation of the MLCK-P-MLC Signaling Pathway
Figure 5
EcNsup inhibited the increased expression of MLCK and the phosphorylation of MLC induced by TNF-α/IFN-γ. (a) The RNA of monolayers was harvested after incubation for 48 h in the absence or presence of 10 ng/mL TNF-α and 10 ng/mL IFN-γ with or without 100 ng/mL EcNsup, and the expression level of MLCK mRNA was detected by RT-qPCR. (b, c) The total protein of monolayers was harvested after treatment as described in (a), and the expression level of proteins was detected by western blot assays. Results were expressed as the (). vs. control. # vs. TNF-α/IFN-γ.
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