The Liver X Receptor Is Upregulated in Monocyte-Derived Macrophages and Modulates Inflammatory Cytokines Based on LXRα Polymorphism

Figure 1

Monocyte-derived macrophage differentiation upregulates liver X receptor α (LXRα) expression. (a) Untreated THP-1 cells (A) and THP-1 cells treated with PMA for 72 h (B). (b) Untreated U937 cells (A) and PMA-treated U937 cells after 72 h (B). (c) mRNA expression of LXRα and ABCA1 is increased in monocyte-derived macrophages after treatment with PMA for 72 h. (d) Protein levels of LXRα are increased in monocyte-derived macrophages (THP-1 and U937) after treatment with PMA for 72 h. For immunoblot analysis of LXRα, total cellular proteins were extracted from THP-1 and U937 cells treated with PMA. Data are shown from three independent experiments. Values are the means and SD. vs. controls.

(a)

(b)

(c)

(d)