The Liver X Receptor Is Upregulated in Monocyte-Derived Macrophages and Modulates Inflammatory Cytokines Based on LXRα Polymorphism

Figure 3

Expression of liver X receptor α (LXRα) and proinflammatory cytokines in human monocyte-derived macrophages (THP-1 and U937) according to LXRα promoter genotypes (-1830 T > C). (a) and (b) mRNA expression of LXRα and ABCA1 was increased in monocyte-derived macrophages (THP-1 (a) and U937 (b)) treated with LXR agonist (T0901317 or GW3965) after treatment with PMA for 72 h, and differential expression is shown according to genotype. mRNA expression of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IFN-α, and interleukin-10 (IL-10) was shown in monocyte-derived macrophages treated with LXR agonists after treatment with PMA for 72 h according to LXRα promoter genotypes. (c) Protein expression levels of LXRα, ABCA1, TNF-α, and IFN-γ are shown for monocyte-derived macrophages treated with LXR agonist. For immunoblot analysis of LXRα, ABCA1, TNF-α, and IFN-γ, total cellular proteins were extracted from THP-1 or U937 derived macrophages treated with LXR agonist. Data are shown from three independent experiments. Values are the means and SD. vs. controls.

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