Post-TTM Rebound Pyrexia after Ischemia-Reperfusion Injury Results in Sterile Inflammation and Apoptosis in Cardiomyocytes

Figure 1

Experimental time-temperature protocol. Normothermic OGD/R-injured groups were maintained at 37°C for the duration of the experiment, while TTM groups were cooled to 33.5°C after 3-hour exposure to OGD and maintained during simulated reperfusion for 24 hours. All experimental cooled groups were then rewarmed to and maintained at 37°C. Cooled pyrexia groups were maintained at 37°C for only 2 hours and then along with normothermic pyrexia groups further warmed to 40°C at 29 h after experimental start and maintained for an additional 24 hours. Samples were analyzed directly after OGD (6 h), 2 hours into the early reperfusion phase (8 h), the end of the cooling phase (27 h), 2 hours after rewarming to normothermia (29 h), and then 2, 12, and 24 hours after initiation of pyrexia (31, 41, and 53 h after experimental start, respectively).