Mannan-Binding Lectin Regulates Inflammatory Cytokine Production, Proliferation, and Cytotoxicity of Human Peripheral Natural Killer Cells

Figure 1

MBL directly bound to NK cells. (a) The binding of indicated concentration of bio-MBL or bio-HSA to purified NK cells was determined by FACS analysis (). (b) For competition assays, purified NK cells were preincubated with (thick lines) or without (thin lines) 50 μg/ml mannan, CLR, or CRD of MBL following MBL binding analysis (). (c) MBL binding to NK cells was also determined using ELISA (). (d) 50 μg/ml CLR of MBL pretreatment following MBL binding analysis was also used in ELISA to determine the binding domain of MBL. MFI = geometric mean. For FACS analysis, cells stained with streptavidin-FITC alone served as the control (shaded). Data are presented as (horizontal lines). ; ; , compared to the control group without MBL in (a) and (c); compared to 20 μg/ml bio-MBL alone in (b) and (d).

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