Research Article
Mannan-Binding Lectin Regulates Inflammatory Cytokine Production, Proliferation, and Cytotoxicity of Human Peripheral Natural Killer Cells
Figure 2
MBL regulated the inflammatory cytokine production in NK cells. Isolated human peripheral NK cells were stimulated with 100 U/ml recombinant IL-2 in the presence or absence of indicated concentration of MBL. Protein (a) and mRNA (c) levels of TNF-α and IFN-γ were analyzed by ELISA (, after 24 h incubation only) and qRT-PCR () assays, respectively. (b) Representative FACS plots and statistical analysis are shown for IFN-γ/TNF-α production analysis after 72 h incubation (). Protein (d) and mRNA (e) levels of IL-10 were analyzed by ELISA () and qRT-PCR () assays after 24 h incubation, respectively. Data are presented as (horizontal lines). ; , compared to the group treated with IL-2 alone.
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