Mannan-Binding Lectin Regulates Inflammatory Cytokine Production, Proliferation, and Cytotoxicity of Human Peripheral Natural Killer Cells

Figure 3

MBL treatment reduced NK cell proliferation. CFSE-labeled or unmarked purified NK cells were stimulated with 100 U/ml recombinant IL-2 in the presence or absence of MBL for 5 days. (a) Effects of different concentrations of MBL on the expansion of NK cells were assessed by the CCK-8 assay. (b) For Ki67 measurement, cells were fixed and stained by anti-Ki67, followed by FACS analysis. (c) Representative division histogram (left panel) and the percentages of CFSE-labeled NK cells in each peak of division (G, generation) (right panel) were calculated by FACS analysis. (d) The cell apoptosis was analyzed by FACS. The percentages represent the frequency of the cells undergoing early apoptosis (Annexin V⁺7-AAD^-) or late apoptosis (Annexin V⁺7-AAD⁺). Data are presented as (horizontal lines) (). ; ; , compared to the group without MBL protein.

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