Mannan-Binding Lectin Regulates Inflammatory Cytokine Production, Proliferation, and Cytotoxicity of Human Peripheral Natural Killer Cells

Figure 5

MBL impaired cytotoxicity of NK cells. The cytotoxicity of NK cells (LAK activity) toward K562 cells (a), expression of CD107a (b), and TNF-α/IFN-γ (c) were evaluated by FACS after coculture of stimulated NK cells and K562 cells with the additional 4 hours, K562 cells alone as the control; CFSE-labeled NK cells were stimulated with 100 U/ml recombinant IL-2 in the presence or absence of 20 μg/ml MBL for 5 days. The percentages represent the frequency of CD107a⁺ NK cells, and the numerical values represent the MFI of CD107a in NK cells (b). MFI = geometric mean. ; , compared to the group with IL-2 alone. Data are presented as (horizontal lines) ().

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