Mannan-Binding Lectin Regulates Inflammatory Cytokine Production, Proliferation, and Cytotoxicity of Human Peripheral Natural Killer Cells

Figure 6

MBL inhibited IL-2-induced activation of STAT5 in NK cells. Purified human peripheral NK cells were stimulated with recombinant 100 U/ml human IL-2 in the presence or absence of 20 μg/ml MBL. Cell lysate was harvested at the indicated time point and then analyzed using immunoblotting for phosphorylation of STAT5 and total STAT5. GAPDH served as a loading control. The representative image of at least three independent experiments with similar results was shown.