Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells

Figure 2

OSS activates TLR4 signaling. (a) TLR4 expression in carotid arteries and CD31 was used to identify endothelium. (b) Analysis for intensity of florescence. (c) Representative immunoblots for TLR4 in OSS-treated cells, the level of proteins was normalized by GAPDH. (d) Bar diagram showing quantitative data of immunoblots. (e) Analysis for mean intensity of florescence. (f) Representative images of ROS-positive cells in static-cultured cells, LPS-treated cells, OSS 30 min-treated cells with or without the preincubation of TAK-242 (1 μmmol/L). ^# versus sham, ; versus control group, versus OSS 30 min, .

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