Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells

Figure 3

OSS activates NOX2. (a) Antioxidant effect of apocynin (10 μmmol/L), allopurinol (1 mmol/L), tempol (1 mmol/L), and rotenone (100 μmmol/L) against OSS-induced oxidative stress. (b) Percentage of ROS-positive cells. (c) Representative immunoblots for NOX1, NOX2, and NOX4 in OSS-treated cells. (d) Densitometry analysis of immunoblots for NOX1, NOX2, and NOX4. (e) Immunoblots of membrane p47. (f) Densitometry analysis of immunoblot for p47. (g) Coimmunoprecipitation of p47 and NOX2 in whole cell lysate. (h) Bar diagram showing quantitative analysis of p47. ^& versus OSS 0 min, ^# versus OSS 30 min, ; versus OSS 0 min, .

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