Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells

Figure 6

Interrelation among TLR4, NOX2, and caveolin-1 in OSS-treated cells. (a) Efficacy of siRNA against TLR4. (b) TLR4 inhibition decreases NOX2 expression with OSS exposure. (c) TLR4 inhibition suppresses the association of eNOS and caveolin-1. (d) Efficacy of siNOX2. (e) TLR4 level following NOX2 inhibition. (f–h) Quantitative analysis of immunoblots. (i) Expression of TLR4 and NOX2 with methyl-β-cyclodextrin treated or not. (j) Bar diagram showing quantitative data of immunoblots. (k) NO and SOD level in ECs with or without TLR4 inhibition under OSS conditions. versus OSS 0 min, ^# versus OSS 30 min without siTLR4 or methyl-β-cyclodextrin, .

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