Matrix Metalloproteinase-9-Dependent Release of IL-1β by Human Eosinophils

Figure 5

MMP-9 activity is involved in IL-1β release by activated eosinophils. (a) Blood eosinophils were cultured with TNF-α plus IL-3 (10 ng/ml) for 48 h after a 30 min treatment with Z-WEHD-FMK (caspase-1 (CASP1) inhibitor) or MMP-9 inhibitor 1 (MMP-9 inhibitor) used at 5 μM or vehicle alone (-). The amount of matured IL-1β in the supernatant culture was measured by ELISA. indicates that MMP-9 inhibition significantly reduced IL-1β compared to no treatment (-) (, different subjects (Table E3)). (b) The THP-1 cell line was differentiated into macrophages using PMA and IFN-γ overnight. Cells were treated with caspase-1 inhibitor (CASP1), MMP-9 inhibitor (MMP9), and vehicle alone (-), the same as for blood eosinophils in (a), and were activated with LPS (100 ng/ml) for 24 h. Matured IL-1β released by THP-1 cells was measured by ELISA. indicates that caspase-1 inhibition significantly reduced IL-1β release compared to (-) and MMP-9 (, ).

(a)

(b)