A Benzenediamine Analog FC-99 Drives M2 Macrophage Polarization and Alleviates Lipopolysaccharide- (LPS-) Induced Liver Injury

Figure 1

FC-99 inhibited the expression of M1 phenotypes induced by LPS and promoted M2 marker expression induced by IL-4. RAW264.7 cells were cultured in 10 ng/ml LPS for 12 h to establish M1 or in 10 ng/ml IL-4 for 24 h to establish M2 macrophage, respectively. (a, b) For the identification of M1 and M2 phenotypes, M1 (iNOS and TNF-α (a)) and M2 markers (Arg-1 and CD206 (b)) were tested using Q-PCR. (c, d) RAW264.7 cells were stimulated by LPS with or without FC-99 (50 μM) for 12 h; mRNA levels of iNOS (c) and TNF-α (d) were determined by Q-PCR. (e, f) RAW264.7 cells were stimulated by IL-4 with or without FC-99 (50 μM) for 24 h; mRNA levels of Arg-1 (e) and CD206 (f) were determined by Q-PCR. Data were presented as the . , , and vs. the indicated group.

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