Production of Soluble Receptor Activator of Nuclear Factor Kappa-Β Ligand and Osteoprotegerin by Apical Periodontitis Cells in Culture and Their Modulation by Cytokines

Figure 2

The levels of RANKL (a), OPG (b), and values of RANKL/OPG ratio (c) in PL cell cultures in relation to clinical characteristics of the lesions and T/B cell predominance. Periapical lesion (PL) cells were isolated from 43 PLs and cultivated for 24 hours as described in “Materials and Methods.” The levels of soluble RANKL and OPG in supernatants were measured by ELISA, and after that, the RANKL/OPG ratio was calculated. The differences between groups were tested by using the pair Student -test. The statistically significant difference was marked on the graph. , , and , as indicated.

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