Production of Soluble Receptor Activator of Nuclear Factor Kappa-Β Ligand and Osteoprotegerin by Apical Periodontitis Cells in Culture and Their Modulation by Cytokines

Figure 3

The levels of RANKL (a), OPG (b), and RANKL/OPG ratio (c) in PL cell cultures upon stimulation with pro- and anti-inflammatory cytokines. Periapical lesion (PL) cells were isolated from 12 PLs and cultivated for 24 hours with TNF-α, IFN-ϒ, IL- 17A, IL-4, and IL-10 as described in “Materials and Methods.” The levels of soluble RANKL and OPG in supernatants were measured by ELISA, and after that, the RANKL/OPG ratio was calculated. (a) Friedman test (, Friedman statistics 22.1, ) and Dunn’s multiple comparison test, and vs Ctrl. (b) Friedman test (, Friedman statistics 15.94, ) and Dunn’s multiple comparison test, vs Ctrl. (c) Friedman test (, Friedman statistics 21.14, ) and Wilcoxon signed-rank test, compared to Ctrl.

(a)

(b)

(c)