Production of Soluble Receptor Activator of Nuclear Factor Kappa-Β Ligand and Osteoprotegerin by Apical Periodontitis Cells in Culture and Their Modulation by Cytokines

Figure 5

Modulation of OPG production in PL cell cultures by RANKL or anti-RANKL neutralizing antibody. Periapical lesion (PL) cells were isolated from 12 PLs and cultivated for 24 hours with 10 ng/ml (labeled as 1 on x axis), 30 ng/ml (labeled as 2) of RANKL, or anti-RANKL neutralizing antibody (2 μg/ml) (labeled as 3) as described in “Materials and Methods.” The levels of OPG in supernatants were measured by ELISA, and after that, the RANKL/OPG ratio was calculated. All differences in relation to control (Ctrl) were not statistically significant ().