Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-I/PI3K/Akt Pathways

Table 1

Primers used for the quantitative real-time PCR.

Primers	GenBank number	Sequence (5-3)	Size (bp)
β-Actin	NM_007393.5	F: CGTTGACATCCGTAAAGACC	111
		R: CTAGGAGCCAGAGCAGTAATC
MCP-1	NM_011333.3	F: CCCTAAGGTCTTCAGCACCT	118
		R: ACTGTCACATGGTCACTCC
TNF-α	NM_013693.3	F: GTGCCTATGTCTCAGCCTCT	129
		R: CTGATGAGAGGGAGGCCATT
IL-6	NM_031168	F: AGACTTCCATCCAGTTGCCT	113
		R: CAGGTCTGTTGGGAGTGGTA
IGF-I	NM_010512.5	F: ACTGGAGATGTACTGTGCCC	105
		R: GATAGGGACGGGGACTTCTG
IGF-IR	NM_010513	F: TCCCCAACCTCACAGTCATC	146
		R: GTCGGCGTTCTTCTCAATCC
IGFBP-5	NM_010518	F: CGAGATGGCTGAAGAGACCT	112
		R: CTGGGTCAGCTTCTTTCTGC

MCP-1: monocyte chemoattractant protein-1; TNF-α: tumor necrosis factor-α; IL-6: interleukin-6; IGF-I: insulin-like growth factor-I; IGF-IR: insulin-like growth factor-I receptor; IGFBP-5: insulin-like growth factor binding protein-5.