Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis

Figure 1

Immunoreactivity for sEH in fetal membranes from normal pregnant women and women with pregnancies complicated by acute CAM. Immunostaining of CK7 delineates the localization of amniotic epithelium and trophoblast cells in choriodecidua (a, b). Compared to normal pregnancy, fetal membranes from women with acute CAM had increased infiltration of monocytes/macrophages, as demonstrated by positive anti-CD68 immunostaining, within the amnion and choriodecidua (d, e). In normal pregnancy, immunostaining of sEH was observed in the amniotic epithelium (g). The immunostaining of sEH was generally more intense, both in the amnion and choriodecidua, in women with acute CAM compared with that in normal pregnant women (h). There was no staining observed in the negative controls when the primary antibody was substituted with nonimmune IgG (c, f, and i). . AE, amniotic epithelium; CH, choriodecidua; arrows, endothelium.